摘要
用ABC免疫组化方法,对30例肝细胞性肝癌(HCC)及其癌旁组织、15例慢性活动性肝炎(CH)穿刺活检组织石蜡标本进行了突变型p53蛋白和HBxAg表达的检测。结果发现:在HCC和癌旁组织中,突变型p53阳性和HBxAg均阳性者分别为13例(43.3%)和15例(50%);突变型p53阳性、HBxAg阴性者分别为5例(16.7%)和8例(26.7%);突变型p53阴性、HBxAg阳性者分别为4例(13.3%)和1例(3%)。卡方检验结果,P值均小于0.05。在15例CH中,有3例出现突变型p53蛋白阳性染色,2例出现HBxAg阳性染色。这些结果表明:突变型p53蛋白与HBxAg存在着明显的相关性,提示HBV感染与p53基因突变密切相关,p53基因突变可能是HBV致肝癌作用的机制之一。
Abstract In order to investigate the expression of mutant p53
protein (Mp53)andHBxAg in chronic active hepatitis(CH)and hepatocellular carcinoma (HCC),30
specimens of HCC with surroundingliver tissus(SL),15 biopsy specimens from CH were
examined with immunohistochemical method(ABC system).The results showed,that
13(43.3%)specimens of HCC and 15(50%) of SL were posi-tive for MP53 and HBxAg staining;
3(16.7%)of HCC and 8(26.7%) of SL were ouly MP53 posi-tive,being HBxAg staining negative. On
the contrary, 4(13.3%)of HCC and 1(3%)of SL werenegative for MP53 staining and positive for
HBxAg staining(P<0.05,χ2 test). In 15 specimens ofCH, 3 cases were positive for MP53,2 for
HBxAg.The results indicate that there is a correlationbetween mutant p53 protein and
HBxAg,suggesting that the p53 gene mutation may be closely re-lated with HBV infection,and
the mutation of p53 gene would be one of hepatocarcinogenesis mech-anisms of HBV.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1994年第3期184-187,T008,共5页
Chinese Journal of Oncology