慢性乙型肝炎病毒感染与肝细胞性肝癌中突变型p53蛋白的表达

EXPRESSION OF MUTANT p53 IN CHRONIC HBV INFECTION AND HEPATOCELLULAR CARCINOMA

用ＡＢＣ免疫组化方法，对３０例肝细胞性肝癌（ＨＣＣ）及其癌旁组织、１５例慢性活动性肝炎（ＣＨ）穿刺活检组织石蜡标本进行了突变型ｐ５３蛋白和ＨＢｘＡｇ表达的检测。结果发现：在ＨＣＣ和癌旁组织中，突变型ｐ５３阳性和ＨＢｘＡｇ均阳性者分别为１３例（４３．３％）和１５例（５０％）；突变型ｐ５３阳性、ＨＢｘＡｇ阴性者分别为５例（１６．７％）和８例（２６．７％）；突变型ｐ５３阴性、ＨＢｘＡｇ阳性者分别为４例（１３．３％）和１例（３％）。卡方检验结果，Ｐ值均小于０．０５。在１５例ＣＨ中，有３例出现突变型ｐ５３蛋白阳性染色，２例出现ＨＢｘＡｇ阳性染色。这些结果表明：突变型ｐ５３蛋白与ＨＢｘＡｇ存在着明显的相关性，提示ＨＢＶ感染与ｐ５３基因突变密切相关，ｐ５３基因突变可能是ＨＢＶ致肝癌作用的机制之一。

Abstract In order to investigate the expression of mutant p53 protein (Mp53)andHBxAg in chronic active hepatitis(CH)and hepatocellular carcinoma (HCC),30 specimens of HCC with surroundingliver tissus(SL),15 biopsy specimens from CH were examined with immunohistochemical method(ABC system).The results showed,that 13(43.3%)specimens of HCC and 15(50%) of SL were posi-tive for MP53 and HBxAg staining; 3(16.7%)of HCC and 8(26.7%) of SL were ouly MP53 posi-tive,being HBxAg staining negative. On the contrary, 4(13.3%)of HCC and 1(3%)of SL werenegative for MP53 staining and positive for HBxAg staining(P<0.05,χ2 test). In 15 specimens ofCH, 3 cases were positive for MP53,2 for HBxAg.The results indicate that there is a correlationbetween mutant p53 protein and HBxAg,suggesting that the p53 gene mutation may be closely re-lated with HBV infection,and the mutation of p53 gene would be one of hepatocarcinogenesis mech-anisms of HBV.