摘要
应用抗p53单克隆抗体及快速免疫组化染色法检测了50例石蜡包埋乳腺癌及7例乳腺良性增生性疾病p53蛋白的表达,结果显示12例(24%)乳腺癌呈阳性反应,7例良性病变均为阴性、在一组原发及淋巴结转移乳腺癌配对标本中,同一病人两个部位p53表达差异无明显意义,提示p53基因异常发生在肿瘤转移之前。在与临床病理指标的相关分析中发现,p53蛋白异常表达与乳腺癌淋巴结转移呈非常显著的相关关系(P<0.005),与髓样癌亦显著相关,4例髓样癌中,2例有淋巴结转移,p53蛋白强阳性。结果表明:p53蛋白不仅与乳腺癌发生有关,而且在乳腺癌淋巴结转移中起重要作用,因此,检测p53蛋白的表达可作为临床预测乳腺癌淋巴结转移趋势的重要参考指标之一。
Abstract The monoclonal antibody to p53 and a rapid
immunohistochemical method were used in thisstudy to detect p53 expression in 50
paraffin-embedded breast cancers and 7 benign hyperplasticdiseases of breast, The results of
immunostaining showed that 1 2 (24%) cases of breast cancers werepositive and all benign
lesions were negative. No significant difference was found between primaryand metastatic
sites of 11 paired samples of breast cancrs in p53 exprecssion, suggesting that the ab-normal
expression of had p53 occurred before lymph node metastasis of breast cancer. Statistical
analysisrevealed that the abnorrnal expression of p53 was significantly correlated with lymph
nodemetastasis of breast cancer(P<0.005) and also correlated with medullary breast cancer in
which twoof four cases strongly positive to p53 had lymph node metastasis. Our results
indicate that the abnormal expression of p53 is not only involved in thedevelopment,but also
involved in the metastasis of breast cancer. Therefore, detection of p53 expres-sion may have
significant clinical lmportance in the evaluation of the tendency of lymphaticmetiastasis of
breast cancer .
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1994年第4期266-268,T010,共4页
Chinese Journal of Oncology