绵羊GDF9和BMP15基因多态性检测

Detection of the Polymorphisms of GDF9 and BMP15 Genes in Sheep

以绵羊GDF9基因FecGH突变和BMP15基因FecXB和FecXG突变为候选基因,采用PCR-RFLP方法研究其在湖羊、夏洛来、陶赛特、萨福克、中国美利奴肉用多胎品系、中国美利奴羊和罗米丽羊7个品种中的多态性.结果发现,湖羊群体中存在GDF和BMP15基因的FecGH和FecXB突变,但发生率极低,分别为0.645%(2/310)和0.968%(3/310);而其它品种中则没有发现GDF9和BMP15基因的相应突变.这一发现对于建立绵羊基因标记辅助选择方法具有重要意义.

Polymorphism of mutation in FecGH of GDF9 gene and mutation in FecX B and FecXG of BMP15 gene were studied by forced PCR RFLP method as candidates fo r prolific genes in 7 breeds or strains of sheep such as Hu,Chinese Merino meat Prolificacy strain,Suffolk,Dorset,Charolais,China Merino and Romney Hills.The results showed that the mutations of FecGH and FecXB were only detected in Hu sh eep,and mutation ratio were 0.645%(2/310)and 0.968%(3/310)respectively,no mutations were detected in other sheep breeds or strains.This finding has signif icance in sheep breeding and early selection assisted by gene markers.