桃仁油不皂化物中甾醇和三萜醇的分离与鉴定

Isolation and Identification of Sterols and Triterpene alcohols from Peach Seed Oil

利用快速硅胶柱层析和制备薄层层析分离桃仁油不皂化物,并通过气相色谱、气相色谱-质谱联用技术以及红外、质谱、核磁等数据分析并鉴定了Δ7-豆甾烯醇乙酸酯,β-谷甾醇乙酸酯,菜油甾醇乙酸酯,豆甾醇乙酸酯和Δ5-燕麦甾醇乙酸酯5种甾醇乙酸酯化合物,以及羽扇醇乙酸酯、24-亚甲基环阿屯烷醇乙酸酯2种三萜醇乙酸酯化合物。

The Sterols and Triterpene alcohols were separated from the unsaponifiable matters in the peach seed oil by flash chromatography with silica gel H and preparative thin-layer chromatography with silica gel-silver nitrate. GC、GC-MS、IR、MS and HNMR were used to identify 1 Δ7-stigmastenol acetate, β-sitosterol acetate, campesterol acetate, stigmasterol acetate, Δ5-avenasterol acetate, Lupeol acetate and 24-methylenecycloartanol acetate.