摘要
目的:探讨荧光定量聚合酶链式反应(FQ-PCR)方法检测尿液中人巨细胞病毒(HCMV)对儿童HCMV活动性感染的检测价值。方法:对收集的175份临床标本分别用FQ-PCR方法及低基质磷酸化蛋白(pp65)抗原血症检测方法进行平行检测比较。结果:71例临床患者尿液FQ-PCR检测阳性59例;血液pp65抗原检测阳性53例。尿液中HCMVD-NA检测结果与HCMVpp65抗原血症的一致性为84·2%。结论:FQ-PCR检测敏感性要高于pp65抗原血症检测,可以有效提示体内病毒的活跃程度,具有良好的应用价值。
Objective:To discuss human cytomegalcvieus action infection in children by quantitative PCR assay in urine.Methods:175 clinical speciments were tested by FQ-PCR and pp65 antigenemia assay at the same time.Results:59 positive speciments were tested in 71 clinical urines samples by FQ-PCR.And 53 cases were tested by HCMV pp65 antigenemia assay. The concordance of two methods was 84.2% .Conclusion:FQ-PCR is more sensitive than pp65 antigenemia assay. It is more effective for detecting HCMV action infection and observed its development, and can be widely used in clinical detection.
出处
《现代预防医学》
CAS
北大核心
2005年第7期740-741,共2页
Modern Preventive Medicine