应用荧光定量PCR技术检测小儿尿液中人巨细胞病毒

DETECTION OF HUMAN CYTOMEGALOVIRUS INFECTION BY FQ-PCR TECNIQUE IN CHILDREN'S URINE.

目的:探讨荧光定量聚合酶链式反应(FQ-PCR)方法检测尿液中人巨细胞病毒(HCMV)对儿童HCMV活动性感染的检测价值。方法:对收集的175份临床标本分别用FQ-PCR方法及低基质磷酸化蛋白(pp65)抗原血症检测方法进行平行检测比较。结果:71例临床患者尿液FQ-PCR检测阳性59例;血液pp65抗原检测阳性53例。尿液中HCMVD-NA检测结果与HCMVpp65抗原血症的一致性为84·2%。结论:FQ-PCR检测敏感性要高于pp65抗原血症检测,可以有效提示体内病毒的活跃程度,具有良好的应用价值。

Objective:To discuss human cytomegalcvieus action infection in children by quantitative PCR assay in urine.Methods:175 clinical speciments were tested by FQ-PCR and pp65 antigenemia assay at the same time.Results:59 positive speciments were tested in 71 clinical urines samples by FQ-PCR.And 53 cases were tested by HCMV pp65 antigenemia assay. The concordance of two methods was 84.2% .Conclusion:FQ-PCR is more sensitive than pp65 antigenemia assay. It is more effective for detecting HCMV action infection and observed its development, and can be widely used in clinical detection.