结合态磷化氢在厌氧微生物产酸过程中的释放行为

Fate of Matrix-Bound Phosphine During Acidification with Anaerobic Bacteria

通过动态实验分别研究了在产甲烷和酸化阶段厌氧污泥中结合态磷化氢的变化,并采用静态厌氧实验进一步阐明在酸化阶段有机酸的积累对磷化氢的影响及消失速率.结果表明,产甲烷阶段,厌氧颗粒污泥磷化氢浓度随高度升高呈下降趋势;酸化阶段,当pH达到4～5时会导致厌氧反应器颗粒污泥中结合态磷化氢消失.静态模拟实验研究表明,随着酸化程度的增加,厌氧污泥中结合态磷化氢消失速率加快.厌氧污泥在葡萄糖浓度2000 mg·L-1培养2 d后,结合态磷化氢浓度从最初的1.76 ng·kg-1降低到0.09 ng·kg-1,磷化氢消失速率最高达到0.84 ng·(kg·d)-1;而葡萄糖浓度400mg·L-1培养2 d后,结合态磷化氢消失速率仅为0.27 ng·(kg·d)-1;随着进一步培养,当磷化氢浓度降低到一定程度后,消失速率也相应下降.

Matrix-bound phosphine of anaerobic granular sludge was studied during methanogenic and acidogenic stage of anaerobic reactors. Results showed that at methanogenic stage, the concentration of matrix-bound phosphine of anaerobic sludge decreased as the height of reactor increased. But during acidogenic stage, matrix-bound phosphine disappeared when pH of the solution declined to 4～5. Further anaerobic static experiments showed that increased acidification and lower pH value promoted the decrease of matrix-bound phosphine. After cultivation with 2 000 mg·L~ -1 glucose synthetic wastewater for two days, the matrix-bound phosphine fell from 1.76 ng·kg~ -1 to 0.09 ng·kg~ -1; At the same time, the highest disappearing rate of phosphine was reached 0.84 ng·(kg·d)~ -1. While the glucose synthetic wastewater was 400 mg·L~ -1,the disappearing rate of phosphine was only 0.27 ng·(kg·d)~ -1. With further cultivation under acidogenic stage, the phosphine decreasing rate slowed down.