大叶秦艽的组织培养与植株再生

Tissue Culture and Plantlet Regeneration of Gentiana macrophylla

以秦艽的叶和下胚轴为外植体,成功地诱导出愈伤组织和再生植株.诱导愈伤组织最合适的培养基为附加2 m g·L- 1 2 ,4 - D和0 .5 mg·L- 1 6 - BA的MS培养基,诱导率可达到10 0 % .愈伤组织转移到附加2 mg·L- 12 ,4 - D和0 .5 mg·L- 1 KT和5 0 0 m g·L- 1 L H的MS培养基上进行继代培养,增殖后的愈伤组织转移到附加0 .1mg·L- 1 2 ,4 - D和0 .5 m g·L- 1 6 - BA的MS分化培养基上进行分化,其分化率可达到86 .6 7% ,将分化出的芽转接到不加激素的MS上,结果可生长出大量的分化苗.

With the leaves and hypocotyls of Gentiana macrophylla Pall., as the explants, callus and regenerated plants were successfully induced. The optimal medium for callus induction was the MS medium with 2 mg·L -1 2,4-D and 0.5 mg·L -1 6-BA added, and its inductivity rate amounted to 100%. The induced callus was transferred to the MS medium with 2 mg·L -1 2,4-D, 0.5 mg·L -1 KT and 500 mg·L -1 LH added for its secondary culture. The callus from the secondary culture was transferred to the MS medium with 0.1 mg·L -1 2,4-D and 0.5 mg·L -1 6-BA added to differentiate, and its differentiation rate could reach 86.67%. The buds differentiated from the callus were transplanted to the MS medium without hormones and then developed into many differentiation seedlings.