银杏叶提取物对糖尿病大鼠心肌线粒体的保护作用

Protective effects of extract gingko biloba on myocardium mitochondria of experimental diabetic rats

目的观察银杏叶提取物(EGb)对糖尿病大鼠心肌线粒体的保护作用。方法40只SD大鼠随机分为正常对照组、糖尿病组和EGb治疗组,观察各组大鼠心肌线粒体超微结构,检测心肌线粒体琥珀酸脱氢酶(SDH)、超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)的活性,以及一氧化氮(NO)、丙二醛(MDA)的含量。结果糖尿病大鼠心肌线粒体主要表现为肿胀,嵴变短,空泡化;SDH、SOD活性下降,NOS活性及MDA、NO含量增高。EGb治疗组病变较糖尿病组明显减轻,心肌线粒体SDH、SOD活性升高,NOS活性及NO、MDA含量下降。结论EGb能保护自由基和过量一氧化氮对心肌线粒体的损伤,提高SDH活性,从而对糖尿病大鼠心肌起保护作用。

ObjectiveTo observe the protective effects of extract gingko biloba (EGb) on the myocardium mitochondria of diabetic rats.Methods40 Sprague-Dauley rats were divided randomly into the normal control group, diabetic group and EGb treatment group. The morphologic changes of myocardium mitochondria were studied by electron microscope, and the activities of succinate dehydrogenase (SDH), superoxide dismutase (SOD), nitric oxide synthase (NOS) and content of nitric oxide (NO), malondialdehyde (MDA) in the myocardium mitochondria were assayed by spectophotometer respectively.ResultsThe expansion of mitochondria, shorten of mitochondrial crest were observed under transmission electron microscope in diabetic rats. The activities of SOD and SDH decreased, but that of NOS and the content of NO increased in the diabetic group compared with control group. In EGb treatment group, the morphological change was slight, the activities of SOD and SDH were increased as well as NOS and the content of NO, MDA decreased compared with the diabetic group.ConclusionEGb can protect myocardium mitochondria of experimentally diabetic rats from lesion of free radical and excessive NO, and enhance the activity of SDH, protect myocardium of diabetic rat consequently.