大鼠脊髓全横断损伤模型的建立

A COMPLETE REDUCTIONISTIC RAT SPINAL CORD TRANSECTION MODEL

目前用于脊髓全横断的动物模型,常遗有部分未损脊髓组织,局部脊髓损伤范围很大,尤其是破坏了的血运扩大了继发性变性,造成了复杂的局部病理变化,致使难以分析治疗性脊髓伤区内移植的疗效。因此迫切需要一种还原论式的脊髓横断模型,本实验设计了一种切割辅以吸除的方法,可以在局部造成平均约0.6mm的清洁横断区,同时保存脊髓腹、背动脉及脊髓背静脉的大鼠脊髓横断模型,并用H.E.染色法、神经丝(NF)和胶质纤维酸性蛋白(GFAP)的免疫组织化学方法及生物素化的葡萄糖胺(BDA)的追踪方法检验损伤区及其两侧脊髓的组织反应。

The spinal cord transection models currently used usually spare a part of the spinal cord; cause large and severe trauma; jeopardize the blood circulation, which enhances secondary degeneration and complexity of the local pathological changes. All these made the analysis of the therapeutic value of various intraspinal-lesion transplantation difficult. Thus reductionistic spinal cord transaction models are in urgent need. We reported here a rat complete transection model by cutting supplemented with suction. We were able to produce a clean cross section lesion of about 0.6 mm, meanwhile preserve the integrity of the ventral and dorsal spinal arteries and the dorsal spinal vein. H.E. staining, BDA tracing technique, NF and GFAP immunohistochemistry were used to examine the local lesion, the completeness of the transection, and the tissue reactions of the nearby spinal cord.