复方肝毒清对免疫性肝损伤模型小鼠的保护作用

Protective Effect of Compound Ganduqing on Mice Liver Injury Induced by Concanavalin A

[目的]观察复方肝毒清对刀豆蛋白A(ConA)所致小鼠肝损伤的保护作用。[方法]昆明种小鼠60只,随机分为空白对照组,模型对照组,肝毒清高、中、低剂量组(20、10、5g/kg),联苯双酯组(150mg/kg);除空白对照组外,所有小鼠2次尾静脉注射ConA(20mg/kg)复制肝损伤模型,各组均按设计剂量灌胃给药,空白对照组和模型对照组给予等容积生理盐水,共给药3次;取血清检测谷丙转氨酶(ALT)、肿瘤坏死因子-α(TNF-α),取肝组织检测TNF-αmRNA表达,并进行肝组织病理学检查。[结果]肝毒清高、中、低剂量组均能降低血清ALT、TNF-α水平,抑制肝组织TNF-αmRNA表达,与模型对照组比较具有显著性差异(均P<0.01),并能一定程度改善肝组织病理变化。[结论]复方肝毒清对ConA所致小鼠肝损伤具有一定的保护作用。

[Objective] To observe the protective effect of the Compound Ganduqing (CG) on mice liver injury induced by concanavalin A (ConA) . [Methods] Sixty Kunming mice were randomly divided into six groups: blank control group (group A), model group (group B), 150 mg/kg biphenyldicarboxylate group (group C), 20g/kg CG group (group D), 10 g/kg CG group (group E), and 5 g/kg CG group (group F). Except the blank control group, the mice were administered with injection of ConA, bid, into the caudal vein to establish liver injury models and then were administered by gavage according to the experiment design. The blank control and model groups were given the same dose of saline, tid. After treatment, serum alanine aminotransferase (ALT) and tumor necrosis factor (TNF-α) levels, TNF-α mRNA expression in the liver tissue and hepatic pathological changes were tested. [Results] Compared with the model group, CG in three dosages markedly depressed serum levels of ALT and TNF-α, inhibited TNF-α mRNA expression (P < 0.01) and improved hepatic pathological features. [ Conclusion ] CG has a protective effect on mice liver injury induced by ConA.