高效液相色谱法测定益心片中阿魏酸和丹酚酸B的含量

Content Determination of Ferulic Acid and Salvianolic Acid B in Yixin Tablets by HPLC

[目的]建立益心片中阿魏酸和丹酚酸13的含量测定方法。[方法]采用高效液相色谱法(HPLC),样品以100倍甲醇为溶媒,超声30min,在C18柱上以乙腈-磷酸(体积分数为0.05%)水溶液为流动相梯度洗脱,于波长320nm处检测。[结果]阿魏酸在0.02432-0.72960μg线性关系良好,r=0.9999,丹酚酸B在0.4168-12.5040μg线性关系良好,r=0.9999,p平均回收分别为100.95%、99.35%。[结论]本法灵敏准确,简便易行,重现性好。

[Objective] To establish a method for the determination of ferulic acid and salvianolic acid B contents in Yixin Tablets. [Methods] High pressure liquid chromatography (HPLC) was adopted. The chromatographic conditions were: 100-fold methanol as solvent, Bonification for 30min, gradient elution on C18 comlumn with acetonitrile and phosphoric acid as mobile phase, the detection wavelength being 320nm. [Results] Ferulic acid had a good linearity in the range of 0.024 32 - 0.729 60 μg and r=0.999 9; salvianolic acid B had a good linearity in the range of 0.416 8-12.504 0μg and r = 0.9999; the average recovery rate was 100.95% in ferulic acid and 99.35% in salvianolic acid B respectively. [Conclusion] This method is simple, accurate and with good reproducibility.