黏附分子CD44v6蛋白表达及DNA含量与乳腺浸润性癌的相关性研究

CORRELATION BETWEEN CELL ADHESION MOLLECULES CD44v6 EXPRESSION, DNA CONTENT AND INVASIVE BREAST CARCINOMA

目的研究CD44v6蛋白表达及DNA含量在浸润性乳腺癌侵袭、转移和预后中的作用。方法应用免疫组化S-P法检测103例浸润性乳腺癌石蜡切片中肿瘤细胞CD44v6的表达,并用图像分析法进行DNA含量分析。结果CD44v6主要定位于细胞质,与乳腺浸润性癌组织学的类型无关(P>0.05),而与组织学的浸润程度有关。CD44v6蛋白在组织学分级:Ⅰ级阳性表达率为57.9%,Ⅱ级为87.0%,Ⅲ级为88.1%(P<0.01)。乳腺浸润性癌DNA异倍体情况:采用Feulgen染色示细胞核呈紫红色,胞浆不着色。DNA图像分析结果显示CD44v6蛋白表达阳性者肿瘤细胞异倍体率为82.3%(65/79),表达阴性者肿瘤异倍体为45.9%(11/24),经2检验有统计学意义(P<0.05)。各组织学类型之间异倍体比较,无显著性差异(P>0.05)。结论CD44v6蛋白表达与DNA异倍体水平呈平行关系。CD44v6、DNA倍体的联合检测对于指导临床医生的诊断具有重要的意义。因此,结合DNA含量和CD44v6蛋白表达的联合检测,对乳腺浸润性癌的诊断、判断预后价值更大。

Objective To study the correlation between CD44v6 expressions, DNA heteroploid and invasive breast carcinoma. Methods 103 cases of breast carcinoma were studied by immunohistochemical technique ,Feulgen stain and automatic image analysis technique, respectively. Results The positive rates of CD44v6 was 76.7% (79103) and they were irrelevant with histological type in invasive breast carcinoma (P>0. 05). The expression of CD44v6 and E-cad were related to the pathological grade of breast cancer (P<0.01).The positive rate in the first grade was 57.9%, in tne second grade was 87.0%, in the third grade was 88.1%, respectively.The study of DNA heteroploid in infiltrating breast cancer indicated that with an increase in degree of malignant tumor, not only did the level of DNA heteroploid increased in various degree, but also the number of heteroploid increased. Conclusions The expression of CD44v6 can be taken as a reference marker of prognosis in invasive breast carcinoma. The expression of CD44v6is parallel with DNA heteroploid. It is better for doctor to direct diagnosis, screen of high-risk patients and prevention to detect CD44v6 associated with DNA heteropolid.