摘要
从马铃薯中提取、纯化了羧肽酶抑制剂(CPI),免疫家兔后产生CPI抗血清。用氯胺-T法制备125I-CPI,采用双抗体聚乙二醇混合法建立了CPI放射免疫分析方法,批内和批间RSD分别为10.2%和7.4%,检测灵敏度为1.5μg/L,平均回收率104%,ED50为16.4μg/L。
Carboxypeptidase Inhibitor (CPI) was isolated and purified from potato, and the antiserum againstCPI as obtained by immunizing the rabbits. 125I-CPI was prepared using the chloramines-T method , and the radioimmunoas-say of CPI was established by the second antibody and PEG method. The intra- and inter-RSD of CPI-RIA were 10.2% and7.4%, respectively. The sensitivity was 1.5μg/L, the average recovery was 104%, and ED50 was 16.4μg/L.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2005年第7期402-404,411,共4页
Chinese Journal of Pharmaceuticals
