锌和硒拮抗二氧化硅细胞毒性作用的研究

Effect of Zinc Gluconate and Sodium Selenite in Antagonism to the Toxicity of Silica in Alveolar Macrophages

目的研究葡萄糖酸锌和亚硒酸钠单独及联合拮抗二氧化硅对肺泡巨噬细胞的毒性作用。方法采用肺灌洗法获得肺泡巨噬细胞(1×106/ml),与二氧化硅同时加入不同浓度的葡萄糖酸锌、亚硒酸钠以及葡萄糖酸锌+亚硒酸钠溶液,共同培养18h后检测巨噬细胞中丙二醛含量、超氧化物歧化酶的活力以及细胞培养液中乳酸脱氢酶的活力。结果葡萄糖酸锌和亚硒酸钠单独作用,以及两者合用都具有拮抗二氧化硅细胞毒性的作用,两者最佳组合是1.5mg/L葡萄糖酸锌+1.0μmol/L亚硒酸钠。结论本实验证实,葡萄糖酸锌、亚硒酸钠在体外可有效拮抗二氧化硅粉尘的细胞毒性作用,降低肺泡巨噬细胞的丙二醛的含量,降低培养液中乳酸脱氢酶的活力以及升高巨噬细胞的超氧化物歧化酶的活力,两者共同作用更为明显,并找到了最佳剂量组合。

Objective To investigate the effect of zinc gluconate and sodium selenite used either solely or together in antagonism to the toxicity of silica on alveolar macrophages. Methods Alveolar macrophages were cultured with silica,at the s ame time zinc gluconate,sodium selenite and zinc gluconate together with sodium selenite were added respectively.Superoxide dismutase(SOD)and malon dialdehyde(MDA)in alveolar macrophage,and lactic dehydrogenase(LDH)were measured .. Results Zinc gluconate,sodium selenite and zinc gluconate toget her with soldium selenite have effects to antagonize the toxicity of silica on a lveolar macrophages.The most suitable combination of the two substances would be medium dosage of zinc gluconate(1.5 mg/L)with medium dosage of sodium selenite (1.0 μmol/L). Conclusions Zinc gluconate and sodium selenite used both solely or together could reduce the levels of MDA,LDH and increase SOD in alveolar mac rophages exposed to silica.