摘要
对金葡球菌标准菌株(ATCC25923)和3株临床分离金葡球菌进行环丙沙星(CPLX)耐药性诱导,并通过琼脂稀释法测定环丙沙星对各菌株最低抑菌浓度(MIC)。利用改良间氯苯腙羰基氰化物(CCCP)结合荧光分光光度法检测了耐药性诱导菌株及其母株对环丙沙星的摄取情况。CCCP的作用不同程度地增加了各菌株对环丙沙星的摄入量,确证了金葡球菌中外输泵介导对环丙沙星耐药性的存在。达到稳态浓度后,耐药性较强的菌株对环丙沙星摄入量低于耐药性较弱或标准菌株对环丙沙星的摄入量。
<Abstrcat> Resistance against ciprofloxacin was induced in 1 Staphylococcus aureus control strain ATCC25923 and 3 clinical isolates by 1/2 MIC, and minimal inhibitory concentration(MIC) of ciprofloxacin against Staphylococcus aureus was determined with disk diffusion and agar dilution susceptibility tests. Intaking of ciprofloxacin was detected by improved CCCP-fluorospectrophotometry in Staphylococcus aureus strains and their induced resistant strains. CCCP did make the intaking of ciprofloxacin higher in all strains. This corroborated the existance of ciprofloxacin resistance when mediated by efflux pump in Staphylococcus aureus. After reaching concentrations of steady state, dose of intaking of ciprofloxacin in strong resistant isolates is lower than that of weak resistant isolates.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2005年第3期335-338,共4页
Journal of Jilin Agricultural University
基金
国家自然科学基金资助项目(30270999)
