牦牛皮胶的毒理学安全性评价

Toxicological assessment of Yak-skin Gelatin

目的:评价牦牛皮胶的安全性,为其应用提供毒理学安全依据。方法:进行小鼠经口急性毒性试验、大鼠遗传毒性试验及30天喂养试验。结果:①小鼠经口急性毒性试验,雌、雄小鼠经口累计两次灌胃量达15g/kgBW(相当于人群推荐量0.133g/kgBW的113倍),属无毒级。②遗传毒性试验。小鼠骨髓微核试验及小鼠精子畸形试验结果均为阴性。③大鼠30天喂养试验。未发现其对试验大鼠的生长发育、血液学、生化、脏体比及组织病理学有明显不良影响。结论:在试验剂量3.28g/kgBW～15g/kgBW范围内。

Objective :To evaluate the toxicological safety of Yak-skin Gelatin in order to provide some toxicological data for its application. Methods :The following experiments of acute toxicity test through mouth in rats , the genetic toxicity test and thirty days feeding test in mice were performed. Results : It was more than 15g/kgBW, equivalent to 113 times of the recommended dosage , in acute toxicity test through mouth in both male and female rats , which fell into the scale of non-toxicity.The negative results were observed in the genetic toxicity test of micronucleus test of bone marrow cells and sperm shape abnormality test in mice. The negative impacts on the growth , development ,hematology , biochemistry , histology and the ratio of organ to whole body were not found on the experimental rats of thirty days feeding. Under the same dosage range , no teratogenic effects were found in the experiments of teratogenicity test . Conclusions:Yak-skin Gelatin can be used with toxicological safety under the experimental dosage range of 3.28g/kgBW～15 g/kgBW.