人参二醇组皂苷对小细胞肺癌细胞增殖的影响及其机制

The Mechanism of Inhibition of Proliferation of Small Cell Lung CarcinomaCell Lines Induced by Panaxadiolsaponins

目的:探讨人参二醇组皂苷对小细胞肺癌细胞增殖的影响及其机制。方法:在小细胞肺癌细胞的培养液中加入不同浓度(50～250mg/L)的人参二醇组皂苷,培养一定时间后,通过测定其中氚标胸腺嘧啶脱氧核苷酸掺入量(3H-TdR),及以MTT法来评定小细胞肺癌细胞增殖程度。同时,测定细胞培养液中超氧化物歧化酶(SOD)、脂质过氧化物水平,以反映细胞氧化还原状态。结果:随着人参二醇组皂苷浓度的增加,3H-TdR及MTT测定的OD值逐渐减少,细胞增殖率逐渐增加,SOD水平逐渐增加,丙二醛(MDA)水平逐渐减少。当人参二醇组皂苷浓度为150,200,250mg/L时,各指标与对照组(未用药)相比均具有显著性差异(P<0.05)。结论:人参二醇组皂苷可增加SOD水平,减少活性氧自由基的产生,抑制小细胞肺癌细胞的增殖。

Objective:To observe the effect of panaxadiolsaponins on the proliferation of small cell lung carcinoma cell lines and its mechanism. Methods:Panaxadiolsaponins (50,100,150,200,250 mg/L)was added to culture medium containing 10% fetal calf serum respectively. 24 h later,the proliferation of small cell lung carcinoma cells were studied with 3H-thymidine incorporation rate(3H-TdR)and MTT methods.The level of superoxide dimutase(SOD),lipid peroxide in medium were also assayed.Results:3H-TdR and OD value by MTT were significantly reduced,while the level of SOD increased and the level of MDA decreased by 150,200,250 mg/L panaxadiolsaponins.Conclusion:Panaxadiolsaponins can increase SOD,reduce the level of reactive oxygen species.It is one of the mechanism that panaxadiolsaponins inhibits the proliferation of small cell lung carcinoma.