摘要
目的:采用高效液相色谱法(HPLC法)测定复方奥硝唑乳剂中奥硝唑和酮康唑的含量。方法:色谱柱为C18柱(4.6mm×250mm,5μm),流动相为甲醇-水(74∶26),流速为1.00mL/min,检测波长为239nm。结果:奥硝唑在28~140μg/mL、酮康唑在42.4~212μg/mL范围内浓度与峰面积呈线性关系。奥硝唑的平均回收率为98.62%,RSD=0.52%(n=4);酮康唑的平均回收率为98.98%,RSD=0.21%(n=4)。结论:该法可同时测定两种成分的含量,简便、迅速,适用于医院制剂分析。
Objective:Ornidazole and ketoconazole in compound ornidazole emulsion w ere determined with HPLC method.Methods:HPLC method was used with a column of C1 8(4.6 mm×250 mm,5 μm).The mobile phase was CH3OH-H2O(74 ∶26).The flow rate wa s 1.00 mL/min;the calibration curves were linear in the range of 28~140 μg/mL for ornidazole and 42.4~212 μg/mL for ketoconazole. Results:The average recove ries of ornidazole were 98.62%,RSD=0.52%(n=4) and ketoconazole were 98.98%,RSD=0 .21%(n=4),respectively.Conclusion:The method was simple and rapid,it can be used for the assay of two components in compound ornidazole emulsion,which is suitab le for the preparation in hospital.
出处
《中国药业》
CAS
2005年第7期40-41,共2页
China Pharmaceuticals