禽畜粪便污染地面水样经氯化消毒处理后的致突变性

STUDY ON MUTAGENICITY OF MANURE-CONTAMINATED SURFACE WATER SAMPLES AFTER TREATMFNT OF CHLORINATION

报道了上海郊区受禽畜粪便严重污染的地面水样经氯化消毒处理后的致突变性。检测方法采用ＴＡ９８、ＴＡ１００菌株回复突变试验（Ａｍｅｓ试验）。水样处理采用树脂柱上吸附，有机溶剂洗脱法。研究结果表明：未经氯化处理的水样，仅少数样品具有微弱致突变性。经氯化处理后，极大多数水样转化为具有较强的致突变性，其中多数不加Ｓ９ｍｉｘ代谢活化系统样品的致突变性强度高于加代谢活化系统的样品。针对研究结果，讨论了与氯化有关的致突变物之可能来源。

This paper reported the mutagenictity of manure-contaminated surface water samples after treatment of chlorination in Shanghai suburbs.These water samples were seriously contaminated by domestic animal excreta. The revertants of salmonella strains TA98 and TA100 were used as indices for detecting the mutagenic potential for such samples,which were prepared by using XAD2 resin for adsorbing The water mutagenic agents and using special organic reagents for eluting the adsorbed components from the resin. The results of detection showed that before chlorination, only a few water samples gave weak mutagenic response to salmonella strains TA98 or TA100, however,after chlorination, the majority of water samples gave an apparent mutagenic response to TA98 or TA100 or both. The mutagenic intensity was higher in those samples without S9 mix supplementation in the detecting system than in those samples with S9 mix supplementation. The possible origin of the mutagenic agents resulting from water chlorination was discussed around the results of detection.