左向右分流大鼠肺血管超微结构的动态变化及血浆硫化氢含量观察

Observation of Dynamic Changes in Ultra-Micro-Structure of Pulmonary Arteries and Endogenous Hydrogen Sulfide in Rats with Left-Right Shunt

目的探讨左向右分流大鼠肺血管超微结构的动态变化及与内源性硫化氢(H2S)的关系。方法经下腔静脉-腹主动脉穿刺术建立左向右分流动物模型,分别在分流术后1、3d,1、4、8周各实验时间点处死大鼠,制作肺组织电镜标本,并用敏感硫电极法测量大鼠血浆H2S含量。结果肺血管超微结构动态变化为进行性加重,首先为内皮细胞改变,然后为平滑肌细胞,最后出现细胞外基质异常堆积。分流组大鼠血浆H2S含量在分流术后4周与对照组比较明显增高。结论左向右分流大鼠肺血管超微结构动态变化中伴随内源性H2S变化,提示内源性H2S可能在肺血管结构改变中发挥调节作用。

Objective To explore the relationship between dynamic changes in ultra-micro-structural of pulmonary arteries and endogenous hydrogen sulfide in rats with left-right shunt.Methods Rats in shunt group were subjected to an abdominal aorta-inferior vena cava shunt to create an animal model of pulmonary artery structural remodeling. After 1 day, 3 days, 1 week, 4 weeks and 8 weeks of experiment, the ultra-micro-morphologic changes of pulmonary arteries of rats were observed under electronic microscope and H_2S concentration in serum was evaluated by modified sulfide electrode method.Results The changes of ultra-micro-structure of pulmonary arteries were progressively exacerbated, endothelial cells became swollen and large in size on 3 days, smooth muscular cells increased in size as well as the change of endothelial cells in 1 week, and they changed from contractile phenotype to synthetic phenotype in 4 weeks.Conclusions Shunt exhibited changes of ultra-micro-structure of pulmonary arteries are accompanied by the changes of endogenous H_2S. It is suggested that endogenous H_2S might play a protective role in changes of ultra-micro-structure of pulmonary artery.