前列腺癌组织中骨桥蛋白及基质金属蛋白酶-2检测

Expression of osteopontin in prostate cancer and its correlation with matrix metalloproteinase-2

目的:检测前列腺癌组织中骨桥蛋白(OPN)及基质金属蛋白酶-2(MMP-2)的表达。方法:对前列腺癌、良性前列腺增生与正常前列腺组织标本,应用半定量RT-PCR检测OPNmRNA的表达,应用免疫组织化学技术检测OPN和MMP-2蛋白的表达。结果:46例前列腺癌组织中OPNmRNA的表达阳性率为76.1%(35/46),OPN及MMP-2蛋白的表达阳性率分别为71.7%(33/46)和67.4%(31/46),两者表达呈正相关(P<0.01)。并且两者在前列腺癌不同分期和分级之间差异有统计学意义(P<0.05)。前列腺增生及正常前列腺组织中2者均未见阳性表达。结论:OPN阳性表达可能与肿瘤的浸润转移有关,OPN与MMP-2可能在前列腺癌的进展中起协同作用。

Aim: To investigate the expression of osteopontin(OPN) in prostate cancer and its relationship with matrix metalloproteinase-2(MMP-2). Methods: The expression of OPN mRNA was detected using semi-quantitative RT-PCR,and the expression of OPN and MMP-2 protein,by S-P imunohistochemical assay. Results: The positive expression rates of OPN mRNA was 76.1%(35/46). The positive expression rates of OPN and MMP-2 were 71.7% (33/46) and 67.4% (31/46) in prostate cancer tissue, respectively, and the expression of OPN was significantly correlated with MMP-2 expression (P< 0.01 ). The expressions of OPN and MMP-2 were related to the tumor grade and clinical stage (P<0.05). Conclusion: OPN may play an important role in the progression of prostate cancer and is a potential marker for invasion and metastasis of prostate cancer. OPN and MMP-2 might play synergetic roles in prostate carcinogenesis.