阿霉素联合TRAIL对人神经胶质瘤H4细胞增殖的影响

Effects of TRAIL and doxorubicin on proliferation of H4 glioma cells

目的:探讨阿霉素联合TRAIL对人神经胶质瘤H4细胞增殖的影响。方法:应用MTT法检测200μg/LTRAIL、100g/L、200g/L、300g/L阿霉素及2者联合应用时H4肿瘤细胞的生长抑制率,以及联合应用MabDR5和(或)zIETD-fmk时的生长抑制率。结果:阿霉素与小剂量TRAIL联用能显著提高H4细胞的生长抑制率;应用Mab-DR5不能有效抑制TRAIL和阿霉素联用时对H4细胞的增殖抑制协同作用,而zIETD-fmk则可以。结论:小剂量阿霉素与TRAIL联合应用具有协同作用,可抑制H4细胞的增殖。

Aim: To study whether doxorubicin could increase the H4 glioma cell death induced by TRAIL and investigate the underlying mechanism. Methods: Using MTT assay to measure the cell growth inhibiting rate of H4 glioma induced by doxorubicin at different concentrations,TRAIL (200 g/L),or both of them, respectively and to detect whether the application of MabDR5 and zIETD-fmk independently or symphysically could inhibit the death induced by combination of doxorubicin and TRAIL. Results: The cell growth inhibiting rate of H4 glioma induced by low-dose TRAIL could be increased by doxorubicin at the concentration of 50% lethal dose. Such synergistic effect of doxorubicin and TRAIL could not be inhibited by MabDR5 but could be significantly inhibited by zIETD GAAB2 fmk. Conclusion: Combination of doxorubicin and TRAIL at low concentration could cause synergistic cytotoxicity and inhibit the proliferation of H4 glioma cells.