摘要
目的研究骨保护素(OPG)和核因子κB受体活化因子(RANK)及其配体(RANKL)在类风湿关节炎(RA)患者外周血和滑液中的表达,探讨RANKL、RANK和OPG在RA骨破坏中的意义。方法收集活动期RA患者治疗前后外周血、滑液和健康对照,以间接免疫荧光标记,流式细胞术检测RANK、RANKL和OPG在外周血和滑液中CD3+T淋巴细胞、中性粒细胞和CD14+单核细胞的表达率及平均荧光强度。结果①RA组治疗前后外周血RANK、RANKL和OPG在CD3+T淋巴细胞、中性粒细胞和CD14+单核细胞上的表达率差异无统计学意义(P>0.01);RANK的表达强度治疗后高于治疗前(P<0.01)。②RA组外周血CD3+T淋巴细胞上RANKL及RANK的表达率和表达强度明显低于滑液,OPG的表达则高于滑液(P<0.01),RANKL/OPG比率明显增高(P<0.01)。结论RANK表达强度的改变提示其可能与RA炎症的发展和转归有关,而活化的CD3+T淋巴细胞可能通过上调RANK、RANKL的表达,下调OPG表达参与RA破骨细胞的分化和骨损伤的调节机制。
Objective To investigate the expression of receptor activator of NF-?_κB (RANK), receptor activator of NF-?_κB ligand (RANKL) and osteoprotegerin (OPG) on monocytes, lymphocytes and neutrophils in peripheral blood and synovial fluid obtained from rheumatoid arthritis (RA) patients, and discuss the role of RANK, RANKL and OPG in bone destruction of RA. Methods The expression rate and intensity of RANK, RANKL and OPG on lymphocytes, neutrophils and monocytes in peripheral blood before and after treatment and synovial fluid before treatment of 10 active RA as well as in peripheral blood of 10 normal healthy controls were studied by indirect immunfluorescence flow cytometry. Results ① The expression rate of RANKL, RANK and OPG in RA before and after treatment had no significant difference (P>0.01); but the intensity of RANK expression in peripheral blood after treatment was higher than that of before (P<0.01); ② On RA CD3+T cells, the expression rate and intensity of RANK and RANKL in peripheral blood were lower than synovial fluid whereas the OPG expression was higher. The ratio of RANKL/OPG heightened markedly (P<0.01). Conclusion The change of intensity of RANK suggests that it may be involved in the development and prognosis of RA inflammation, and activated CD3+T cells may play an important role in osteoclastogenesis and bone destruction in RA by up-regulating the expression of RANKL and down-regulating that of OPG.
出处
《中华风湿病学杂志》
CAS
CSCD
2005年第7期417-420,共4页
Chinese Journal of Rheumatology
