岱衢族大黄鱼种质的AFLP分析

IDENTIFICATION OF GERM PLASM IN PSEUDOSCIAENA CROCEA TAI-CHU RACE BY AFLP

根据耳石年轮确定作为研究材料的浙江海区捕捞野生大黄鱼为岱衢族大黄鱼,并用AFLP技术进行种质分析。结果表明岱衢族大黄鱼可分为两类:岱衢族大黄鱼Ⅰ型和岱衢族大黄鱼Ⅱ型,这种划分与文献报道的岱衢族大黄鱼有“春综”和“秋综”两种群之分相吻合。岱衢族大黄鱼Ⅰ型生长速度比岱衢族大黄鱼Ⅱ型要快,海水养殖开发和利用岱衢族大黄鱼应选用岱衢族大黄鱼Ⅰ型作为亲本较好。获得的岱衢族大黄鱼种质AFLP指纹图谱,可以作为鉴定岱衢族大黄鱼的依据。

Pseudosciaena crocea,local name Large yellow croaker,is a very important economic fish in China coast.There are three geographical populations(races)from north to south of China coast.In Zhejiang sea area,the indigen is P. crocea Dai-chu race.Because the artificial weif of non indigen P.crocea Min-Uehtung race,the indigen in Fujiang sea area,in zhejiang sea area from 1998,the individuals captured from this area not always belong to the indigen, P.crocea Dai-chu race.But the individuals born before 1996 must be the P.crocea Dai-chu race.Cross section of the otoliths was used to identify the age of wild Large yellow croaker captured from the year 2001 to 2002 from Zhejiang sea area.31 out of 100 individuals captured in 2001—2002 were identified to be the P.crocea Dai-chu race,the youngest was 4 year old;the oldest was 16 year old. The germ plasm analysis by AFLP(Amplified fragment-length polymorphism)suggested that the P.crocea Dai-chu race could be divided into two types,one was type Ⅰ of P.crocea Dai-chu race,and another was type Ⅱ of P.crocea Dai-chu race.This partition was accordant with the literature that the Large yellow croaker in East China sea consisted of two populations,Spring Population and Autumn Population.And they were different from reproductive period.The type Ⅰ of P.crocea Dai-chu race grows quicker than the type Ⅱ.So the type Ⅰ should be chosen as the parents in mariculture of P.crocea Dai-chu race.The AFLP fingerprint achieved by this investigation could be used to identify P.crocea Dai-chu race.