O139群霍乱弧菌地方分离株的分子特征研究

Molecular characteristics of the local isolates of Vibrio cholerae serogroup 139

目的研究本地分离出的O139群霍乱弧菌的分子特征,建立霍乱暴发和散发的快速检测及流行病学溯源的有效手段。方法采用PCR方法对霍乱弧菌进行4种毒力基因的检测,用随机扩增多态性分析(RAPD)及SPSS软件对以上菌株进行多态性分析,对霍乱弧菌毒力进行快速测定和分子分型。结果5株O139群霍乱弧菌均可检出四种毒力基因。所有霍乱弧菌的RAPD结果经聚类分析可分为3个聚类群,较好地反应了不同群的霍乱弧菌之间的亲缘关系。结论可将PCR和RAPD方法结合分析用于霍乱疫情的快速检测和流行病学溯源。

The molecular characteristics of Vibrio cholerae serogroup 139 was investigated in order to search for a rapid detection method of cholera outbreak and the effective means of conducting epidemiological source -tracking. PCR was adopted to detect 4 virulence genes of V.cholerae and the random amplified polymorphic DNA (RAPD) and SPSS software were utilized to conduct DNA polymorphism analysis of the local isolates so that the rapid detection and molecular subtyping of these isolates became possible. It was found that 4 virulence genes were detected from each of the 5 isolates of V.cholerae serogroup 139, and all the isolates could be classified into 3 groups by RAPD, reflecting the relationship of different serogroups of V.cholerae. In conclusion, it is evident that PCR,together with RAPD can be used for the rapid detection of epidemic situation and for the epidemiological source-tracking.