食管癌HSD17B4基因表达及其杂合性丢失分析

Altered Expression of the HSD17B4 Gene in Esophageal Squamous Cell Carcinoma and Loss of Heterozygosity Analysis

目的探讨HSD17B4基因在食管癌中的变化及其可能的意义。方法采用逆转录聚合酶链式反应(RT-PCR),分析HSD17B4基因mRNA表达状态,同时运用PCR银染技术,检测HSD17B4基因内微卫星标记D5S1384在食管癌组织基因组DNA水平的缺失。结果40例食管癌及其癌旁组织标本微卫星标记D5S1384分析发现,26例为信息个体,12例肿瘤组织存在D5S1384的杂合性丢失,丢失率为46.2%。HSD17B4基因mRNA表达下调的比例为62.5%(25/40)。结论HSD17B4可能是5q23区域内的候选食管癌抑制基因。

Objective To investigate the alteration of the gene HSD17B4 in esophageal squamous cell carcinoma and its potential significance. Methods The mRNA expression and loss of heterozygosity(LOH)of HSD17B4 in 40 primary esophageal tumors were detected by reverse transcriptase-polymerase chain reaction(RT-PCR)and microsatellite analysis with the intragenic marker D5S1384 of the gene. Results The frequencies of allelic loss of D5S1384 and the rate of down-regulation of gene HSD17B4 were 46.2% and 62.5%, respectively. Conclusion HSD17B4 may be a candidate tumor suppressor gene associated with esophageal squamous cell carcinoma.