TRAIL及NF-kB在骨肉瘤中的表达及其意义

Expression and Significance of TRAIL and NF-kB in Osteosarcoma

本文探讨TRAIL及NFkB在骨肉瘤中的表达及其与细胞增殖调控的关系。对16例非化疗骨肉瘤,5例骨巨细胞瘤和6例软骨肉瘤石蜡切片,应用流式细胞术(FCM)定量检测TRAIL及NFkB的表达。结果表明TRAIL和NFkB在骨肉瘤各期间的表达显著高于骨巨细胞瘤和软骨肉瘤(P<0.01);TRAIL和NFkB在骨肉瘤不同分化组间表达无明显差异(P>0.05);TRAIL和NFkB在骨巨细胞瘤和软骨肉瘤间表达无明显差异(P>0.05)。结论是TRAIL在骨肉瘤中高表达,但可能受NFkB高表达的调控,不能诱导细胞凋亡;在骨肉瘤的细胞增殖调控中,NFkB可能通过调控TRAIL诱导的凋亡途径进而抑制细胞凋亡的发生。

To investigate the relationship between expressions of TRAIL, NF-kB and cell proli- feration in human osteosarcomas, the expressions of TRAIL and NF-kB in 16 cases of osteosarcoma,5 cases of giant cell tumor of bone and 6 cases of chondrosarcoma were studied by flow cytometry. The expressions of TRAIL and NF-kB in osteosarcomas of different differentiation states were higher than those in other two kinds of tumors significantly in our study(P<0.01). There was no significantl difference between osteosarcomas with different differentiation states(P>0.05). The expressions of TRAIL and NF-kB in chondrosarcoma and giant cell tumor of bone were not different significantly(P>0.05). The higher expression of TRAIL in osteosarcoma with different differentiation states could not induce apoptosis because of the higher expression of NF-kB. NF-kB may restrain the apoptosis of tumor cells by regulating the NF-kB- induced apoptosis path way in osteosarcoma.