仿生脉冲电磁场对去卵巢大鼠骨质疏松治疗作用及机理的初步探讨

The therapeuetic effect of bionics electromagnetic fields in OVX-induced osteoporosis rats and its mechanism

目的观察仿生电磁场对去卵巢大鼠骨质疏松的治疗作用,探讨其作用机理。方法6月龄雌性未孕Wistar大鼠40只,按体质量随机分为模型组(OVX)、假手术组(Sham)、仿生电磁场治疗组(EM)、雌激素治疗组(E)。OVX、EM、E组行双侧卵巢切除术,Sham组行假手术。术后8周,E组苯甲酸雌二醇肌肉注射,0.5mg/kg,1次/2周。EM组大鼠暴露于仿生电磁场治疗,1h·1次-1·d-1,OVX、Sham组不予以任何处理,作为对照组。治疗10周后测量腰椎骨密度、椎体的最大载荷、右侧胫骨骨结构以及血尿生化。结果仿生电磁场显著改善了大鼠腰椎骨密度(P<0.01)、最大载荷(P<0.01),骨结构也明显改善。血尿生化显示骨形成大于骨吸收。结论仿生电磁场对去卵巢大鼠骨质疏松具有明显的治疗作用,增强成骨细胞功能,抑制破骨细胞功能是其治疗作用的可能机理之一。

Objective To investigate the therapeuetic effect of bionics electromagnetic fields and to explore the mechanism of that in postmenopausal osteoporosis.Methods The fourty 6-month old female Wistar rats were randomly divided into four different groups: ovariectomy group(OVX),sham operation group (Sham), E group (Estrogen+ OVX)and EM group(BEMF+ OVX). All rats were subjected to bilateral ovariectomy except sham operation group. After 8 weeks operation,the E group rats were given estrogen 0.5mg/kg,1/2W. EM group rats were exposed to bionics electromagnetic fields, 1h /d. OVX and Sham group rats were given nothing. After the treatments finished,the specimens of left tibia. Results After the treatment with BEMF, BMD at the lumbar spine significantly increased by 24.3% (vs. OVX),the maximum load of EM group markedly significantly increased by 22% (vs. OVX) (P<0.01). The trabecular volume and trabecular connections significantly increased (vs. OVX). The TRAP concentrations in EM group decreased(vs. OVX)(P<0.05),The BGP concentrations, the ALP concentrations increased (vs. OVX)(P<0.05).Conclusion The BEMF are useful in the treatment of postmenopausal osteoporosis resulting from ovariectomy. Building up the osteoblasts and controlling the osteoclasts may be one of mechanisms of therapeutic effect of BEMF on postmenopausal osteoporosis.