UF-100全自动尿沉渣分析仪检测尿液红细胞的应用

Valuation of UF-100 Automatic Flow Urinary Sediment Analysis Bend in Red Blood Cell in Urine

[目的]评价某公司的UF100全自动流式尿沉渣分析仪(下简称UF100)检测尿液中红细胞(RBC)的性能。[方法]按照NCCLSLiteratureGP16A和尿沉渣检查标准化的建议和要求,选择750份临床标本用上述的方法进行分析评价。[结果]UF100计数尿液中红细胞结果与传统的手工牛鲍氏计数板法有良好相关性,γ=0.973,t检验结果P>0.05;RBC<5000个/μl时,有良好的线性与准确性;在RBC>40个/μl时,CV<6%。尿液中的细菌、真菌、草酸钙结晶能对红细胞的测定造成干扰。[结论]UF100是一种尿液中红细胞定量分析快速筛查的方法,建议采用其作为常规的尿沉渣筛查工具。

[Objective]To valucate the performance of the method used in detecting red blood cells(RBC) in urine by Sysmex UF-100 automatic flow urine Analysis(UF-100).[Methods]Collecting 750 cases of clinical urine samples under the requirement of NCCLS Literature GP16-A,analysis by above method.[Results]It has a favorable relativity in detecting of urine RBC by UF-100 and Neubauer’s Counting Board.RBC γ=0.973,t examination(P>0.05);Good linearity in range of 5 000 RBC/μl and high degree.CV<6% when the number of RBC is samller than 40 RBC/μl.The bacteria and candida、have different effects in RBC detection in urine.[Conclusions]UF-100 is a fast and easy testing methond to perform urine sediments quantitative erinalysis.We sugest to choose the Sysmex UF-100 as sieving rule testing RBCs methond.