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RECK基因在前列腺癌组织中的表达及其与基质金属蛋白酶的关系 被引量：6

The expression of the RECK gene in the prostate carcinoma tissues and the relationship with the MMP-2
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摘要 目的:通过检测RECK基因以及基质金属蛋白酶(MMP2、MMP9)在前列腺癌组织及正常前列腺中的表达,探讨RECK基因在前列腺癌发生及转移过程中的作用。方法:抽取组织总RNA,应用RT PCR方法检测RECK及MMP2、MMP9mRNA的表达;抽取组织总蛋白,应用Westernblot方法检测RECK蛋白的表达。结果:前列腺癌组织RECKmRNA的含量较正常组织明显降低(P<0.05),MMP2、MMP9mRNA含量则明显升高,RECK蛋白的表达亦较正常组织明显降低。结论:RECK是一种抑癌基因,可抑制MMP2、MMP9的表达,从而抑制前列腺癌的发生和转移。 Objective:To investigate the effects of the RECK gene in the progression and invasion on the prostate carcinoma by evaluate the expression level of the RECK gene and MMP-2 , MMP-9 in the prostate carcinoma and normal prostate tissues. Methods: The mRNA level of prostate was measured by (RT-PCR) technique and the protein level was evaluated by Western blot. Results:The mRNA level of RECK gene on the carcinoma tissues was lower than that on the normal prostate tissues, while the (MMP-2) , MMP-9 level were higher. The protein level of RECK on the carcinoma was lower than that on the normal tissue. Conclusion:RECK gene was supposed to inhibit the expression of the MMP-2, MMP-9.
作者 徐振宇 高建平 张征宇 葛京平 许传亮 孙颖浩
机构地区 上海第二军医大学长海医院泌尿外科 南京军区南京总医院泌尿外科
出处 《医学研究生学报》 CAS 2005年第7期577-579,598,共4页 Journal of Medical Postgraduates
基金 国家杰出青年基金资助项目(批准号:30225046)
关键词 前列腺癌 RECK基因 基质金属蛋白酶 转移 Prostate carcinoma RECK gene MMP Metastasis
分类号 R737.25 [医药卫生—肿瘤]
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参考文献13

  • 1Bissell MJ, Radisky D. Putting tumors in context[J]. Nat Rev Cancer, 2001,1(1):46-54.
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二级参考文献18

  • 1季润元,冯振卿.KAI1基因与肿瘤侵袭、转移、预后的关系[J].医学研究生学报,2004,17(10):949-951. 被引量：7
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  • 10Wood M, Fudge K, Mohler JL et al. In situ hybridization studies of metalloproteinases 2 and 9 and TIMP-1 and TIMP-2 expression in human prostate cancer[J]. Clin Exp Metastasis, 1997,15(3):246-258.

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医学研究生学报
2005年 第7期

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