结球甘兰自交不亲和系的离体培养繁殖研究

STUDY ON“IN VITRO”PROPAGATION OF SELF-INCOMPATIBLE LINES OF CABBAGE(Brassica oleracea L.var.capitata)

对结球甘兰(Brassica olerasea L.var.capitara)自交不亲和系材料的下胚轴、子叶及花茎切段等进行离体培养快速繁殖和再生植株的遗传稳定性等的探讨。经过连续4代培养后,“黄苗”自交不亲和系材料在 MS+BA(4mg/l)的培基养上获得了12267/90天的繁殖率;经过12代的连续培养后,“上黑”自交不亲和系材料在 MS+BA(2mg/l)的培养基上,获得了84983926/365天的紧殖率,其繁殖系数达常规法繁殖系数的18倍。两种材料均在不含任何激素的 MS 培养基上获得了诱导生根最佳效果。幼苗移栽成活率达100%。离体培养的再生植株具有良好的遗传稳定性。

An“in vitro”technique was developed for the rapid propagationof cabbage self-incompatible lines in the study.Hypocotyls,cotyledonsand young flower shoot segments were used as explants,inoculatedaseptically on MS media containing different combinations of anaphthy-lcetic acid(NAA)and 6-benzylaminopurine(BA).After four succeissivecultures,a 12267 shoots per 90 days rate of multiplication for“Huangmiao”was obtained on the medium with no NAA and 4mg/l BA;andafter twelve successive cultures a 84 983 926 shoots per year rate ofmultiplication for“Shang hei”was obtained on the medium with noNAA and 2mg/l BA.Once plantlets were regenerted in vitro,there washighly genetic uniformity between the regenerated plants obtained afterfour successive cultures and their original lines.It suggested that the“in vitro”propagation system provided by this experiment might besuitable to rapid clonal propagation of Self-incompatible lines ofcabbage.