谷胱甘肽与N-乙酰半胱氨酸对谷氨酸诱导海马神经元损伤的影响

Effect of glutathione and N-acetylcysteine on hippocampal neuron’s damage induced by glutamate

目的观察还原型谷胱甘肽(reducedglutathione,GSH)与N-乙酰半胱氨酸(N-Acetylcysteine,N-NAC)对不同浓度谷氨酸(glutamate,Glu)诱导的海马神经元损害的影响。方法选用新生Wistar大鼠原代培养海马神经元谷氨酸细胞毒性模型,采用台盼蓝活细胞拒染及TUNEL细胞凋亡原位检测等方法比较GSH与NAC对100μmol/L及500μmol/LGlu细胞毒性损伤的影响,并与MK-801比较。结果GSH与NAC能够降低100μmol/LGlu作用下神经元死亡率与凋亡率,NAC组细胞存活率高于同条件下GSH组,其中1mmol/LNAC组神经元存活率达到90.4%±5.2%,与10μmol/LMK-801组相比,差异无统计学意义;在500μmol/LGlu作用下,GSH与NAC则不能增加神经元的存活率,但1mmol/LNAC抗500μmol/LGlu诱导调亡的作用与MK-801相比无明显差异。结论GSH及NAC对轻度Glu细胞毒性神经损伤有保护作用。

Objective To study the effect of reduced glutathione (GSH) and N-acetylcysteine (NAC) on hippocampal neuron death caused by glutamate at different concentrations. Methods Glutamate cytotoxicity model of primary culture neurons from neonatal Wistar rat hippocampal was used to evaluate the effects of GSH and NAC on neuron's damage caused by Glu and compared with that of MK-801.Trypan blue dying and TUNEL labeling were used to evaluate neuron's death and apoptosis respectively. Results GSH and NAC could decrease neuron's death and apoptosis induced by 100μmol/L Glu,and NAC was more efficient in decreasing neuron's damage. There was no statistically difference in the ratio of surviving neurons between 1mmol/L NAC group and 10μmol/L MK-801 group. Both GSH and NAC had no protective effect on neuron's death and apoptosis induced by 500mol/L Glu. Conclusion Glutathione and NAC can protect neurons against light Glu damage.