胰岛素样生长因子Ⅱ和Ki-67在先天性皮肤血管瘤及血管畸形中的表达

Expression of Insulin-like Growth Factor 2 and Ki-67 in Hemangioma and Vascular Malformation

目的探讨胰岛素样生长因子Ⅱ(IGF-Ⅱ)在血管瘤增殖与退化中的作用。方法应用免疫组化方法检测52例血管瘤、27例血管畸形和5例正常皮肤标本中IGF-Ⅱ及细胞增殖核抗原Ki-67的表达情况。同时应用RT-PCR法检测9例血管瘤、5例血管畸形和5例正常皮肤标本中IGF-ⅡmRNA的表达情况。结果免疫组化结果显示血管瘤组的IGF-Ⅱ和Ki-67表达均高于血管畸形组及正常皮肤组(P<0.01)。血管瘤组中增生期亚组两标记指数均高于退化期亚组(P<0.01)。血管瘤组中IGF-Ⅱ与Ki- 67标记指数的相关系数为0.84,P<0.01。RT-PCR法检测到增殖期血管瘤中有明显IGF-ⅡmRNA的表达,而消退期血管瘤、血管畸形和正常皮肤中未见明显IGF-ⅡtuRNA的表达。结论IGF-Ⅱ与血管瘤内皮细胞的增殖相关。

Objective To study the role of insulin-like growth factor 2 (IGF- Ⅱ ) in the proliferation and degeneration of hemangiomas. Methods The expression of IGF- Ⅱ and Ki-67 was detected with SP im-munohistochemical method in 52 specimens of hemangiomas, 27 of vascular malformation and 5 of normal skin. The expression of IGF-Ⅱ mRNA was examined by reverse transcription polymerase chain reaction (RT-PCR) in specimens from 9 cases of hemangiomas and 5 of vascular malformation. Results The labelling indexes (LI) of IGF- Ⅱ and Ki-67 and the expression of IGF- Ⅱ mRNA were significantly higher in heman gioma than those in vascular malformation and normal skin (P < 0.01). LI were also higher in hemangioma at proliferative stage than those at degenerative stage (P < 0.01). There was significantly positive correlation between LI of IGF- Ⅱ and Ki-67 in hemangioma (r = 0.84, P < 0.01). The expression of IGF- Ⅱ mRNA was significantly increased in hemangioma at proliferative stage, and not obvious in hemangioma at degenerative stage, vascular malformation and normal skin. Conclusion IGF- Ⅱ is related to the proliferation of vascular endothelial cells in hemangioma.