摘要
异硫氰酸荧光素(FITC)使C3H小鼠致敏后,其淋巴结中树突状细胞(DC)明显增加,而且均发荧光,电镜下证实为郎格罕氏细胞(LC)样。利用免疫电镜进行DC内FITC超微结构定位,可见大部分免疫金标记分布在细胞核周区和呈丛样定位于溶酶体。金标Thy-1.2抗体只显示在淋巴细胞上。结果证实多数附着素FITC位于抗原呈递细胞内,并与细胞器有关,由此建议附着素的呈递涉及到细胞内化和内处理作用,而不是与细胞表面蛋白的结合。
xposure of C3 H mice to the
contact sensitizer,(Fluorescein isothrocyanate.FITC),was
chara-cterized by an increase in dcndritic cells(DC)in the draining
lymph node(DLN),Many of these DCwere fluorescent and were identified
by electron microscopy as Langerhans cells.The purpose of this
investigation was to identify the fluorescent DC by immunoelectron
microscopy to determine the ultra-stucture localization of FITC. The
majority of the antiFITC label was inside the cells in the
perinucleararea.Gold particles were present in clusters in lysosomes.
Serial sections immunostained with polycIonalanti-thyl.2 antibody
showed gold label only in lymphocytes.These results demon strate that
most hapten is internalized and associated with cytoplasmic
organelles in antigen-presenting cells,suggesting thatpresentaion of
hapten involves action processing and internalization,rather than
binding of hapten tosurface proteins.
出处
《北京医科大学学报》
CSCD
1994年第3期181-183,T009,T01,共5页
Journal of Peking University(Health Sciences)
关键词
接触性过敏
抗原呈递细胞
Contact sensitization,
Antigen presenting cell,lmmunoelectron microscopy