用抗溴脱氧尿苷单克隆抗体检测小鼠各种器官内S期细胞分布的研究

A Study of Distribution of S-Phase Cells in Various Organs of Mice with Antibromodeoxyuridine Antibody

用溴脱氧尿苷（ＢｒｄＵ）腹腔注入正常小鼠（０．１５ｍｇ／ｇ体重），１ｈ后处死，取出多种器官的组织用Ｃａｒｎｏｙ液固定，石蜡包埋切片。用免疫组织化学技术，以抗－ＢｒｄＵ的单克隆抗体检测不同器官内被ＢｒｄＵ标记的Ｓ期细胞。结果证明，ＢｒｄＵ阳性细胞主要分布在细胞增殖活跃的组织，如舌与食管的基底层细胞、胃小凹处的颈粘液细胞，小、大肠的肠腺细胞和睾丸曲细精管内的精原细胞等。ＢｒｄＵ阳性细胞在淋巴器官内主要分布于胸腺皮质、脾脏的动脉周围淋巴鞘和脾小结的生发中心以及淋巴结的生发中心等。高度分化的细胞如舌与食管上皮的角化层或肝、肾等处的细胞呈阴性。

he mice used this study were received intraperitoneal injection of 5-bromodeoxyuridine at adosage of 0.15 mg/g body weight . and the animals were sacrificed by cervical dislocation 1hr lat-er. The various organs were removed and fixed in Carnoy fluid . then dehydrated and embeded inparaffin and cut 5 m thicked sections. Monoclonal antibody specific to 5-bromodeoxyuridine(BrdU), was used to detect BrdU labelling of S-phase cells in mouse various organs. The resultsinditated that BrdU labeled cells are predominantly in the actively proliferating cells, such as thebasal cells of epithelium in tongue and esophagus, mucous neck cells in stomach , epithelium ofcrypts in small and large intestine and spermatogonia in seminiferous tubules of testis. The BrdUlabeled cells in the lymphoid tissues are predominantly located in cortex of thymus, germinal cen-ter of lymph node and Periarterial lymphatic sheath and splenic nodule of spleen. Highly differnti-ated cells are not lateled by BrdU , which included stratum corneum of tongue and esophagus,kidney and liver cells.