氯化汞对水稻细胞悬浮培养植株再生的影响

The Effects of Mercuric Chloride on Plant Regeneration from Rice Cell Suspension Cultures

粳稻品种藤板５号的细胞悬浮培养物，用０．０５％ＨｇＣｌ２处理２分钟，绿芽和绿苗分化率分别提高到８４．１％和２９．７％，用安替福民处理无明显作用。在含活性炭培养基上的预培养时间对悬浮细胞的分化也有较大影响。经ＨｇＣｌ２处理和活性炭吸附的悬浮细胞形成的愈伤组织，其二胺、多胺、内源脱落酸（ＡＢＡ）和玉米素（ＺＲｓ）含量最高，内源吲哚乙酸（ＩＡＡ）含量最低。内源赤霉素（ＧＡｓ）的表现无明显规律性。

Rice cell suspension cultures were established from a Japonica variety,Fujisaka 5.The re- generation frequencies of somatic embryos ,and shoots and plantlets were greatly increased(shoot :84.1%。plantlet :29.7%),after 2 min treatment of 0.05% mercuric chloride and 7-10 day activated charcoal preculture ,and the treatment of Antifomin had no effects on plant regeneration of rice cell suspension culture.The activated charcoal preculture period of mercuric chloride treat- ed cell clusters also influenced the differentiation rate.The callus derived from mercuric chloride and activated charcoal treated cell clusters gave rise to the highest concentration of the total en- dogenous polyamine contents ,diamine-polyamine ratio and endogenous ABA and ZRs contents but the lowest endogenous IAA contents. Precocious germination of somatic embryo resulted in poor pllarity development and low conversion late.