体外驱除瘤胃厌氧真菌对绵羊和山羊细菌、原虫及稻草干物质降解率的影响

EFFECT OF IN VITRO ELIMINATION OF ANAEROBIC RUMEN FUNGI ON BACTERIA AND PROTOZOA POPULATION AND DEGRADATION OF STRAW DRY MATTER IN SHEE PAND GOATS RUMEN

选择体况良好、体重30kg左右、安装有永久性瘤胃瘘管的内蒙古白绒山羊和内蒙古土种去势绵羊各3只作为供体羊,为体外批次培养提供瘤胃液。比较研究了驱除瘤胃厌氧真菌对绵羊和山羊细菌、原虫及稻草干物质降解率的影响。结果表明:对照组(F0组)绵羊瘤胃液中真菌游动孢子数量多于对照组山羊瘤胃液;且在培养的2、4、6、48、72小时差异显著(P<0.05)。在培养初期(0～12小时)山羊F0组和F2组(驱真菌组)细菌总数均高于绵羊;绵羊和山羊驱真菌组(F2组)细菌总数均高于对照组(F0组)。山羊F0组(对照组)原虫数量各时间点的平均数显著高于绵羊F0组(P<0.05)。驱除厌氧真菌后绵羊和山羊对稻草干物质降解率显著下降,分别下降了12.06%和8.62%。这说明瘤胃厌氧真菌在纤维物质降解过程中的确发挥了重要作用。

Three male local sheep and three male Inner Mongolia cashmere goats,age d between 1.5 to 2 years and weighing 25.0 to 32.0kg,with a permanent cannula in the rumen were used to suppley rumen fluid in the study on the effect of in vit ro elimination of anaerobic fungi on bacteria and protozoa population and degrad ation of straw dry matter in the rumen.The results showed the enumeration of fun gi zoospores in rumen liquid phase was much more in sheep than in goats,however, the population of bacteria and protozoa in goats was apparent higher than in she ep.The digestibility of straw dry matter did not differ significantly between th e two species,but was higher in sheep(55.40%)than in goats(53.25%).The results a lso indicated the quantity of bacteria was increased significantly after elimina tion of anaerobic fungi,however,the digestibility of straw dry matter was signif icantly decreased(12.06% and 8.62% for sheep and goats respectively).The results suggested that the rumen anaerobic fungi played a vital important role in the d egradation of dietary fibre.