伪狂犬病病毒Bartha-K61株UL49基因的克隆和序列分析

Cloning and sequencing of the UL49 gene of pseudorabies virus Bartha-K61 strain

根据已经发表的伪狂犬病病毒(PRV)国内Ea株UL49基因序列,设计并合成了1对引物,通过PCR方法扩增到了PRVBartha-K61株UL49基因的编码区,并克隆到pMD18_T载体中。重组质粒pMD_UL49经XhoI酶切和PCR鉴定后,进行了序列测定和分析。结果表明,重组质粒pMD_UL49含有PRVBartha_K61株UL49基因的编码区。同源性分析显示,PRVBartha_K61株UL49基因序列与国外Kaplan株、国内Ea株相应基因的核苷酸同源性分别为98.9%和94.1%,推导的氨基酸序列同源性分别为96.7%和87.2%。

According to the published sequence of UL49 gene of pseudorabies virus ( PRV) Ea strain,a pair of primers were designed to amplify the UL49 gene of pseudorabies virus (PRV) Bartha_K61 strain.The UL49 gene was amplified by polymerase chain reaction (PCR),subsequently cloned into pMD18_T vector.The recombinant plasmid pMD_UL49 was identified by PCR and Xho I restrictive digestion,then sequenced. The seqencing result showed that the recombinant plasmid pMD_UL49 contained the UL49 gene of 750 bp.The nucleotide homology of UL49 gene between Bartha_K61 and Kaplan and Ea strain was 98.9 % and 94.1 %,respectively.The deduced amino acid homology of UL49 gene was 96.7 % and 87.2%,respectively.