大豆黄酮对肉仔鸡生长相关激素水平与免疫机能的影响

Effect of Daidzein on Endocrinology and Immune Function of Broilers

将640只1日龄艾维茵肉鸡公母分养,分别随机分为4组,分别饲喂添加大豆黄酮0、5、10、15mg/kg的4种饲粮,于试验28、49d采血和屠宰,探讨了日粮中添加不同水平大豆黄酮对肉仔鸡生长相关激素水平及免疫机能的影响。结果显示,日粮中添加大豆黄酮28、49d,均可显著提高公鸡血清生长激素(GH)、胰岛素样生长因子-1(IGF-1)、睾酮(TESTO)的含量(P<0.05或P<0.01),显著降低公鸡血清尿素氮水平(P<0.05或P<0.01),而对母鸡血清GH、IGF-1、尿素氮水平无明显影响;添加大豆黄酮49d,显著提高公鸡T淋巴细胞ANAE阳性率(P<0.05或P<0.01),添加大豆黄酮28d,显著提高公鸡NDV抗体效价(P<0.05),而对母鸡无明显影响(P>0.05);添加大豆黄酮28、49d,对公母鸡免疫器官重量有增加的趋势,但差异不显著(P>0.05)。

Six hundred and fourty Avian broilers of 1 day were distinguished female from male and divided into four groups at random respectively.Control group was fed with basal diet,group Ⅰ,group Ⅱ and group Ⅲ was supplemented with 5,10 and 15 mg/kg daidzein in the basal diet respectively.After 28 d and 49 d 12 broilers of each treatment were picked out to be taken blood and then slaughtered,Samples of blood and immune organs were collected for hormones and immunology analysis.Addition of daidzein to diet significantly increased serum growth hormone(GH),insulin-like growth factor-1(IGF-1) and testoterone(TESTO) and significantly decreased serum urea nitrogen in males in 28 d and 49 d,but there was no significant effect on females.T lymphocyte ANAE positive rate of males in 49 d and NDV antibodies level of males in 28 d were significantly improved in daidzein treatments,whereas no significant effect on females.Relative weight of immune organs in males and females were inclined to increase,but there was no significant difference.