摘要
以去势雌兔作为对照,用高胆固醇饮食法复制动脉粥样硬化(AS)模型,研究了利维爱(Livial)在防治兔动脉粥样硬化过程中对雌激素受体和低密度脂蛋白受体mRNA表达的影响。应用RT-PCR方法,β-actin做内参照,对各组去势雌兔心脏、肝脏组织内雌激素受体和低密度脂蛋白受体mRNA的表达进行了检测。结果,2种受体在兔心脏和肝脏内均有表达。对照组,雌激素受体在心脏、肝脏内的相对表达量分别为0.43±0.12、0.39±0.20;低密底脂蛋白受体在心脏、肝脏内的相对表达量分别为0.27±0.05、0.86±0.12,差异显著(P<0.001)。AS模型组,雌激素受体和低密度脂蛋白受体在心脏、肝脏内的相对表达量分别为0.29±0.03、0.27±0.06和0.04±0.01、0.17±0.02,2种受体的mRNA表达均比对照组降低,差异显著(P<0.001)。利维爱组(饲喂高胆固醇饲料同时给予利维爱),雌激素受体在心脏、肝脏内的相对表达量为0.41±0.09、0.36±0.06,与模型组相比表达量显著提高(P<0.001),与对照组表达量接近;低密度脂蛋白受体在心脏、肝脏内的相对表达量为0.04±0.02、0.18±0.04,与模型组比较未见显著变化。由此认为,组织内2种受体的mRNA表达量降低可促进动脉粥样硬化的发生;利维爱通过提高组织内雌激素受体mRNA的表达可防治AS的发生与发展,对低密度脂蛋白受体的mRNA表达影响较小。
To study the effect of Livial on ER and LDLR of atherosclerotic rabbits.Methord:Rabbits oophorectomized and fed with normal feedstaff were used as control group.An atherosclerosis animal model was created with high cholesterol feed.We studied the effect of livial on the expression of estrogen receptor and low-density lipoprotein receptor mRNA in heart and liver with half quantity RT-PCR.Results:The two receptors expressed in heart and liver;the ER mRNA expression in the heart and liver of control were 0.43±0.12,0.39±0.20,the low-density lipoprotein receptor mRNA expression in the heart and liver were 0.27±0.05,0.86±0.12;The estrogen receptor and low-density lipoprotein receptor mRNA expression in the heart and liver of model separately were 0.29±0.03,0.27±0.06 and 0.04±0.01,0.17±0.02.The mRNA expession of the two receptors reduced than that of the control group.In the livial group,the expession of ER in the heart and liver were 0.41±0.09,0.36±0.06 and significantly increased as compared with the model group(P<0.001).The expession of LDLR in the heart and liver were 0.04±0.02,0.18±0.04.Conclusion:The reduce of the two receptors could accelerate the developoment of AS;Livial could protect against the occuration and development of AS by increasing the mRNA expression of ER.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2005年第4期417-419,共3页
Chinese Journal of Veterinary Science
