摘要
以苯酚为内标物,用反相高效液相色谱法(RP-HPLC)测定了人体晨尿中尿酸(UA)、对-氨基苯甲酸(P-ABA)、马尿酸(HA)、高香草酸(HVA)的含量;探讨了尿样预处理技术.该法的检测限为0.23～0.59ng;回收率为97.65%～98.49%;相对标准偏差为1.05%～2.73%.本文建立的分析方法可满足临床胃癌、肺癌等病人的尿液中有机酸的监测与诊断.
The content of uric acid,p-Aminobenzonic acid,Hippuric acid,Homovanillic acid in human’s urine were determinated by Reversed-phase High-Performance Liquid Chromatography,using phenol as internal standard.The pre-treatment technique of urine was discussed briefly.The recoveries are from 97.65% to 98.49%,and the relative standard deviations are from 1.05% to 2.73%,and the detection limits are from 0.23 to 0.59 ng.The analytical method is suitable for the routine monitoring and diagnosis of organic acid in cancer's urine.
出处
《安徽大学学报（自然科学版）》
CAS
北大核心
2005年第4期75-78,共4页
Journal of Anhui University(Natural Science Edition)
关键词
高效液相色谱法
尿酸
马尿酸
高香草酸
对-氨基苯甲酸
high-performance liquid chromatography
uric acid
hippuric acid
homovanillic acid
p-Aminobenzonic acid
