HPV16E7基因疫苗诱导小鼠细胞免疫功能的实验研究

HPV16E7 DNA vaccine can induce specific cell-mediated immune responese in BALB/c mice

目的:探讨重组HPV16E7质粒免疫小鼠后诱导的细胞免疫反应。方法:将BALB/c实验小鼠随机分为三组,实验组小鼠免疫pcDNA3 1 HPV16E7,对照组Ⅰ小鼠免疫pcDNA3 1,对照组Ⅱ小鼠注射生理盐水。每周免疫一次,共免疫三次。末次免疫后一周,眼眶取血后处死小鼠。体外进行脾淋巴细胞增殖反应;流式细胞仪检测脾细胞亚群比率和外周血CD4 +、CD8+淋巴细胞亚群数量;ELISA法检测脾细胞培养上清和血清中IFN γ的含量。结果:pcDNA3 1 HPV16E7免疫小鼠的脾细胞增殖反应明显高于对照组。CD4 +细胞数、CD4 +/CD8+比值及脾细胞培养上清和血清中的IFN γ含量均高于对照组。结论:pcDNA3 1

Objective:To investigate specific cellular immune response to the HPV16E7 prophylactic vaccine in mice.Methods:BALB/c mice were randomly divided into 3 groups:experimental group (treated with pcDNA3.1-HPV16E7),control group Ⅰ(treated with pcDNA3.1) and control group Ⅱ(treated with N.S.).Mice were injected (i.m.) pcDNA3.1-HPV16E7,pcDNA3.1 and N.S. one time per week,respectively.After three immunization,the blood samples from eye sockets and the supernatant cultured of spleen cells were taken for measurement IFN-γ and the number of CD4 +?CD8 +T-lymphocyte by ELISA and FACS assay.Antigen-specific splenocyte proliferation assay in vitro was detected by MTT method.Results:The splenocytes actively proliferated,the number of CD4 +T lymphocyte and the quantitation of IFN-γ in spleen and serum in the experimental group were significantly higher than the control group Ⅰ and Ⅱ.Conclusion:The pcDNA3.1-HPV16E7 DNA vaccine can induce specific cellular immune response in BALB/c mice.