电针对沙鼠急性脑缺血再灌注后神经原损伤的保护作用

NEUROPROTECTIVE EFFECTS OF ELECTRO-ACUPUNCTURE IN A GERBIL MODEL OF TRANSIENT CEREBRAL ISCHEMIA AND REPERFUSION

本实验利用沙鼠急性脑缺血再灌注模型，研究电针对脑缺血及再灌注各期脑电活动的影响及组织病理学的改变。结果表明：缺血１０ｍｌｎ，脑电幅度受到严重抑制，甚至变平坦，总功率大大下降，再灌注后总功率难以恢复，在１２０ｍｉｎ时仅恢复到缺血前的２７．３９±１１．３１％，以后即不再进一步恢复，电针组动物缺血１０ｍｉｎ再灌注后，脑电的恢复明显比对照组快，１２０ｍｉｎ时恢复至缺血前的７１．４５±１６．４６％（Ｐ＜０．０１），２４０ｍｉｎ时继续恢复至缺血前的７５．２７±１８．４３％。同时电针能明显减轻缺血１０ｍｉｎ后再灌注２４小时的神经原缺血性损伤。结果提示：电针对急性脑缺血引起的神经原损伤具有保护作用，并能促进脑功能的恢复。

In the model of transient cerebral ischemia and reperfusion gerbil, we studied the effects of electru-acupuncture (EA) on EEG at different periods of cerebral ischemia and reperfuslon as well as the histological changes. Our results showed that, after 10 min of ischemia, the amplitude of EEG was severely inhibited, even became flat, and the total power of EEG was severely decreased tO 1.41± 1.29%. After reperfusion, the recovery of total power was very slow. The peak level of recovery occured at 120 min following reperfusion was 27.39 ± 11.31%. In comparson with the control group, The recovery of total pewer was 71.45± 16.46%(P< 0.01), and 75.27± 18.43%(P< 0.01)at 120 min and 240 min after reperfusion respectively. EA also substantially reduced the ischemic neuronal damage after 10 min of cerebral ischemia and 24 h of reperfusion. These results indicate that EA has neuroprotective effects on trasient cerebral ischemia and reperfusion injury of gerbils.