益气养阴解毒通络方对糖尿病大鼠血浆、脑组织神经肽Y和神经降压素的影响

The Influence of Preparation for Supplementing Qi, Nourishing Yin, Relieving Du and Activating Luo on the Neuopeptide Y and Neurotensin in Plasma and Brain Tissue of Diabetes Rats

目的探讨糖尿病并发症的发病机理及益气养阴解毒通络方对其影响。方法采用链脲佐菌素左下腹腔注射所致糖尿病大鼠模型,应用放射免疫方法,分别测定糖尿病大鼠血浆、脑组织神经肽Y(NPY)与神经降压素(NT)水平。结果糖尿病大鼠血浆NPY水平明显高于脑组织;糖尿病大鼠脑组织的NT水平明显高于血浆;糖尿病大鼠血浆和脑组织的NPY水平较正常组显著升高;糖尿病大鼠脑组织NT水平明显低于正常组;中药组大鼠血浆和脑组织的NPY和NT水平与正常组比较均无显著性差异。结论NPY与NT在糖尿病大鼠不同组织器官的分布及其作用是有差别的;影响糖尿病脑血管病变的因素可能与NPY和NT的分泌失调有关;益气养阴解毒通络方具有调节糖尿病大鼠血浆和脑组织NPY和NT水平,从而防治糖尿病性脑血管并发症的作用。

Objective Discuss the pathogenesis of the complications of diabetes and study the influence of the preparation for supplementing Qi, nourishing Yin, relieving Du and activating Luo on the complications. Method The levels of neuropeptide Y (NPY) and neurotensin (NT) in plasma and brain tissue are detected by using the animal model prepared by injection of streptozotocin into left lower peritoneal cavity and employing the radioimmunoassay. Results It shows that the level of NPY of plasma is significantly higher than that of brain tissue, whereas the level of NT of brain tissue is obviously higher than that of plasma in the diabetes rats. Since the level of NPY in diabetes rats is distinctively increased when compared with the normal control rats in the present study, it is likely that the diabetes rats are undergoing the complications of brain vessels. Furthermore, due to the significantly lower level of NT in diabetes rats compared with the normal rats, it is implied that the factors of cerebrovascular diseases are correlated with the secretive disorder of NPY and NT. There is no significant difference in the levels of NPY and NT between the administrated Chinese medicine groups and the normal groups. Conclusion The distribution and effect of NPY and NT in diverse organs and tissues of diabetes rats are different. The preparation for supplementing Qi, nourishing Yin, relieving Du and activating Luo can regulate the levels of NPY and NT, hereby can prevent and treat the cerebrovascular complications of diabetes.