电针刺激诱发Fos、Jun样蛋白在大鼠上丘的表达

EXPRESSION OF FOS-AND JUN-LIKE PROTEIN IN THE RAT SUPERIOR COLLICULUS FOLLOWING ELECTROACUPUNCTURE STIMULATION

大量资料表明，即刻早期基因产物Ｆｏｓ蛋白与Ｊｕｎ蛋自的诱导表达可以做为神经元活动的标志物。本研究利用免疫组化技术观察并比较外周电针刺激（２Ｈｚ，１—２－３ｍＡ，３０ｍｉｎ）后，Ｆｏｓ、Ｊｕｎ样蛋白在大鼠上丘的表达。结果表明，正常大鼠上丘的Ｆｏｓ、Ｊｕｎ表达很低，而电针可使Ｆｏｓ表达增加１０倍，Ｊｕｎ的表达增加１８倍。Ｆｏｓ、Ｊｕｎ样免疫反应阳性神经元在上丘备层的分布基本相似，均以表层分布最为密集，中间层次之，深层最少。阳性神经元计数结果表明，Ｊｕｎ在上丘各层次及各断面的表达高出Ｆｏｓ约一倍。关于电针刺激上丘表层大量Ｆｏｓ、Ｊｕｎ表达功能意义有待进一步研究。

The Fos protein derived from c-fos proto-oncogene has been known to form a heterodimer with Jun protein, which functions as a third messenger to indece the expression of target gene. Immunohistochemical technique was used in the present study to detect and compare Fos and Jun expressed in adjacent sections of the rat superior colliculus (SC) following electroacupuncture (EA) stimulation (2Hz, 1 - 2 - 3 mA, 30min). It was shown that the expression of Fos and Jun in SC was scaty in normal animals. EA stimulatin induced a 10 fold inerease in Fos expression and 18 fold increase in Jun expression. The distribution patterns for the two proteins in SC were similar, with the heaviet labeling found in the superficial layer, moderate in the intermediate layer and the lightest in the deep layer. The number of Jun-like immunoreactive neurons doubled that of Fos - like immunoreactive neurons in all levels and subdivisions of SC. SC has been known to be functionally related to vision, although recent studies suggest it may also be involved in nociception. The functional significance of a dramatic expression of Fos and Jun in SC following EA stimulation remains to be elucidaled.