小麦微卫星引物在克氏针茅和冰草中应用的初步研究

Preliminary Study on Application of Stipa Krylovii and Agropyron Cristatum by SSR Primers Derived from Wheat.

微卫星标记因其具有等位基因丰富、检测技术简单等优点,成为近几年迅速发展的一种分子标记技术。微卫星的一个具有潜在价值的特征,就是从一个物种产生的引物可以应用于相关的分类群。试验尝试利用合成的2对小麦的SSR引物(Xgwm23D、Xgwm323A),通过梯度PCR对克氏针茅和冰草的DNA进行PCR扩增,结果一对引物(Xgwm323A)在两者中均有扩增,初步摸清了小麦微卫星引物(Xgwm323A)在克氏针茅和冰草中PCR反应条件,为进一步研究奠定了基础。

Microsatellites or simple repeat sequence have already demonstrated to be a powerful tool in recent years. They have high level and allelic diversity, which are easily and economically assayed by polymerase chain reaction.Two pairs of wheat’ microsatellite primers` (Xgwm2-3D, Xgwm32-3A) were used to amplify Stipa krylovii and Agropyron cristatum’s DNA by gradient PCR, the result shows a pair of wheat primer(Xgwm32-3A) was amplified in both templets. Meanwhile, the anneal temperature of wheat microsatellite primers’ (Xgwm2-3D, Xgwm32-3A)in Stipa krylovii and Agropyron cristatum were found.