气相色谱法测定蔬菜中残留有机磷农药

Detection of Organic Phosphorus Residues in Vegetable by Gas Chromatography

为了快速测定蔬菜中残留的敌敌畏等6种有机磷农药,用丙酮、二氯甲烷提取出蔬菜中残留的有机磷农药,提取物经DB-17毛细管柱分离后,用配有火焰光度检测器的气相色谱仪进行测定。结果表明,匀浆、抽滤1次,萃取2次(A1B2时效果最好),检测下限达6.08×10-4mg/kg～1.66×10-3mg/kg,6种有机磷农药的回收率范围为82.52%～92.02%,相对标准偏差(RSD)为6.31%～17.06%。本方法符合国家标准对农药残留量测定方法的要求,定性和定量准确,可快速同时测定出蔬菜中残留的敌敌畏、甲胺磷、乙酰甲胺磷、久效磷、甲基对硫磷和毒死蜱等6种有机磷农药。

A quick method for the detection of six kinds of organophosphorus pesticides residues in vegetable has been established in this paper which contains extracting the organophosphorus pesticides from vegetable with acetone and dichloromethane and then separating them through the DB-17 capillary column, and in the end accurately measuring by gas chromatograph with flame photometric detector. The satisfactory result was obtained after one time of suction and two times of extraction (A_1B_2) while the minimum detection limits were from 6.08×10 -4 mg/kg to 1.66×10 -3 mg/kg and the recoveries of organophosphorus pesticides residues ranged from 82.52% to 92.02%, with an average RSD of 6.31%～17.06%.The method is a quick and practical analysis detection method meeting the requirements of national method of pesticides residues analysis with accurate quality and quantity in the detection of six kinds of organophosphorus pesticides in vegetable such as divhlorvos, chlorpyrifos, methamidoophos,parathion-methyl, monocrotophos and acephate.