丙酮酸乙酯对烫伤延迟复苏大鼠脾淋巴细胞增殖及凋亡影响

Effects of ethyl pyruvate on splenocyte proliferation and apoptosis in burn rats with delayed resuscitation

目的观察丙酮酸乙酯对烫伤延迟复苏大鼠脾淋巴细胞增殖及凋亡的影响,并对其机制进行初步探讨。方法72只Wistar大鼠随机分为假烫伤组(n=24)、烫伤组(n=24)和丙酮酸乙酯(3.23mg/ml)治疗组(n=24),分别于伤后1、3和5d活杀大鼠,检测脾淋巴细胞增殖能力及凋亡情况。结果烫伤延迟复苏后1～5d,脾淋巴细胞对丝裂原刺激的增殖反应明显受抑制(P均<0.05);在烫伤后第1d,脾CD3+CD4+T细胞凋亡显著增加(P<0.05)。应用丙酮酸乙酯治疗能够明显恢复烫伤后1～5d脾淋巴细胞的增殖反应(P<0.05),同时烫伤后第1d的凋亡明显下降(P<0.05)。结论丙酮酸乙酯能够有效恢复烫伤延迟复苏后脾淋巴细胞的增殖能力,并减轻脾淋巴细胞凋亡。

Objective To investigate the effects of ethyl pyruvate (EP) on splenocyte proliferation and apoptosis in burn rats with delayed resuscitation, and its potential underlying mechanism. Methods Seventy- two male Wistar rats were randomly divided into shamscalded control group (n=24), scald group ( n=24), and scald with EP treatment group (n=24). Animals were sacrificed on days 1, 3, and 5 postburn, and spleen samples were collected to determine splenocyte proliferation and apoptosis. Results Splenic lymphocyte proliferation response to T cell mitogen, concanavalin A (Con A), as significantly depressed from 1 to 5 days after burn injury (all P<0.05). Meanwhile, burn injury resulted in a marked increase in splenic CD3^+CD4^+T lymphocyte apoptosis in comparison with that in sham-scalded controls on day 1 postburn (P<0.05). Treatment with EP after burns resulted in a dramatic restoration of lymphocyte proliferation response and reduction of splenic CD3^+CD4^+T lymphocyte apoptosis compared with scald group (P<0.05). Conclusion Administration of EP can markedly improve the splenocyte proliferation response and inhibit splenic CD3^+CD4^+T lymphocyte apoptosis in thermally injured rats.