创伤性休克对大鼠肠淋巴液和血液中内毒素肿瘤坏死因子-α和白细胞介素-6的影响

Influence of trauma-hemorrhagic shock on endotoxin, tumor necrosis factor-α and interleukin-6 levels in mesenteric lymph and blood of rats

目的探讨大鼠肠淋巴液在创伤性休克过程中发生细菌移位和炎症反应的可能。方法复制创伤性休克大鼠模型并从肠淋巴干收集淋巴液,比较创伤性休克前后肠淋巴液及血液中内毒素(ET)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的变化。结果淋巴液中ET、TNF-α、IL-6浓度在休克中明显升高(P均<0.05),复苏后除IL-6持续升高至2h外,ET、TN-α均降至正常水平。结论创伤性休克大鼠休克期可从肠淋巴液观察到细菌移位现象,并可能导致TNF、I-L6水平升高,触发全身炎症反应。

Objective To investigate the influence of trauma-hemorrhagic shock on endotoxin (ET), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in mesenteric lymph and blood of rats. Methods Mesenteric lymph from trauma-hemorrhagic shock rats were collected, levels of ET, TNF-α and IL-6 in mesenteric lymph were compared with those from blood samples. Results Levels of ET, TNF-α and IL-6 in mesenteric lymph were elevated during shock period (all P<0.05), and they were decreased to normal range after resuscitation, with an exception of high IL-6 level up to 2 hours following resuscitation. Conclusion The bacterial translocation could occur during shock period via lymphatic pathway, thereby leading to the increase in TNF-α and IL-6 levels, as well as systemic inflammatory response syndrome.