桂枝汤有效部位B对胃肠运动双向调节作用的实验研究Ⅵ—对cAMP、蛋白激酶A和C活性的影响

Experimental Study of Active Fraction B of Guizhi Tang(Cinnamon TwigDecoction)on Dual-Direction Regulation of Gastrointestinal Motility Ⅵ:Effect on cAMP,Protein Kinase A and Protein Kinase C

目的:探讨桂枝汤有效部位B(Fr.B)对胃肠运动双向调节的作用机理。方法:应用放射免疫法测定阿托品致胃肠运动受抑或新斯的明致胃肠运动亢进大鼠下丘脑及胃肠组织cAMP含量、PKA和PKC活性。结果:对阿托品致胃肠运动受抑大鼠,Fr.B可拮抗下丘脑和空肠组织cAMP含量及PKA、PKC活性的降低,对胃窦组织PKA活性的降低也具拮抗作用;对新斯的明致胃肠运动亢进大鼠,Fr.B可升高胃窦组织PKA活性、下丘脑和空肠组织PKC活性,对cAMP含量无明显影响。结论:Fr.B对胃肠运动的双向调节与其影响下丘脑及胃肠局部组织中cAMP含量和蛋白激酶活性有关。

Objective:To investigate the effective mechanism of dual-direction regulation of Fr.B,an active fraction extracted from Guizhi Tang(GZT).on gastrointestinal motility.Methods:The cAMP content,protein kinase A(PKA)and C(PKC)activity of hypothalamus and gastrointestinal tissues in rats with excited or inhibited gastrointestinal motility induced respectively by neostigmine or atropine were measured via radioimmunoassay.Results:Fr.B increased the cAMP content and PKA and PKC activity of hypothalamus and jejunum in rats with inhibited gastrointestinal motility significantly,and antagonized the decrease of PKA activity of gastric antrum.In rats with excited gastrointestinal motility,Fr.B increased PKA activity of gastric antrum,PKC activity of hypothalamus and jejunum,and did not affect the cAMP content.Conclusion:Fr.B could affect the cAMP content,PKA and C activity in hypothalamus and gastrointestinal tissues to regulate gastrointestinal motility in a dual-direction manner.